At the start of a clause or a sentence, conjunctive adverbs should be preceded by a semicolon (or a period) and followed by a comma. The comma is a signal that the adverb modifies (describes) the whole sentence or clause that follows.

Put a comma after the transition word "next" to indicate that it modifies a clause or a sentence, to give directions, or signal the order of steps.

As a synonym of "by the way", use commas to set off the conjunctive adverb "incidentally". When modifying a single word (typically to remark on a coincidence), do not use commas to separate "incidentally" from the word (verb, adjective, or adverb) it describes.

When modifying a single word, do not place a comma before or after "accordingly" to separate it from the verb it describes. At the start of a sentence or a clause, add a comma after "accordingly" to signal that it modifies the whole sentence or clause that follows.

When meaning "nevertheless", add a comma after "still" at the start of a sentence or clause. When using "still" as a regular adverb, to modify a single word, do not use commas to separate "still" from the verb or adjective it describes.

At the beginning of a sentence, transition words such as "of course" and "certainly" are often followed by a comma. Some style guides, however, consider that this comma is not always necessary.

At the start of a sentence, add a comma after the conjunctive adverb "regardless" or the discourse marker "anyway" to introduce a sentence or clause.

At the start of a sentence, using a comma after "meanwhile" or "in the meantime" is optional. Commas are also optional in mid-sentence, but we should use them to signal a strong interruption or include nonessential information.

Add commas after "first", "second", "third", etc. when introducing a series of items. But a comma is generally unnecessary (and sometimes incorrect) when using "first" as an adverb of time, an adjective, or a noun.